Each year of the project we complete sequential extractions for soil phosphorus, iron and mineral sulfide analyses from 17 locations along the Shark River Slough and Taylor Slough/Panhandle transects. The results of these analyses in freshwater, mangrove, and Florida Bay systems will provide important baseline information on the characteristics of the soils from each of the SRS and TS/Ph transects where different partitioning of potential limiting nutrients is expected based on soil type (e.g., peaty versus marly soils), seasonal flooding characteristics (e.g., permanently flooded versus seasonal drawdowns), and/or proximity to the shallow subtidal estuary (e.g. adjacent to coastal shelf versus Florida Bay). The effects of restoration initiatives and/or long-term changes in climate and disturbance can also be tracked by observed changes in soil nutrients over the course of the study.