Florida Coastal Everglades Long Term Ecological Research
Florida Coastal Everglades LTER - Project Information
Project Information

Chemical characteristics of dissolved organic matter in an oligotrophic subtropical wetland/estuary ecosystem

Short-term project
Start date: 2001          End date: 2002
Contact person: Rudolf Jaffe
Funding organization(s):
National Science Foundation NOAA Center for Sponsored Coastal Ocean Research South Florida Water Management District

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The objective of this study was to investigate in detail the molecular characteristics of DOM in a sub-tropical, oligotrophic aquatic environment, namely the Florida coastal Everglades, along a freshwater wetland, mangrove fringe to estuarine transect. For this purpose, surface waters from five sites along Taylor Slough ranging from freshwater marsh to mangrove ecotone environments, as well as three sites within the Florida Bay estuary were collected for analyses. Fluorescence properties of DOM were measured for a quick assessment of DOM quality. Ultrafiltered DOM samples (UDOM; <0.7 ?m, >1000 Da) were concentrated and freeze dried for the determination of bulk C composition, lignin-phenol concentration, and neutral sugar composition. These molecular characteristics were investigated by 13C-NMR spectroscopy, TMAH thermochemolysis, and hydrolysis with trifluoroacetic acid (TFA), respectively. Furthermore, UDOM samples were classified by cluster analysis based on their pyrolysis products obtained via flash pyrolysis-GC/MS.

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National Science Foundation logo This material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DEB-1237517, #DBI-0620409, and #DEB-9910514. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.
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